KARAKTERISTIK EDIBLE FILM PATI TAPIOKA DENGAN PENAMBAHAN MINYAK ATSIRI DAUN JERUK PURUT SEBAGAI ANTIBAKTERI

Abstract

Edible film is a biodegradable packaging that composed from organic compound. Edible film can be
contaminated by pathogen bacteria. Kaffir lime leaves essential oil has able to inhibit bacteria growth. The
aim of the study was to determine the best concentration of kaffir lime leaves essential oil as antibacteria on
tapioca edible film. The experiment research used a completely randomized design, which consist of five
treatments within three replications. The treatmens were concentration of essential oil i.e A1 (0% v/v), A2
(0,5% v/v), A3 (1% v/v), A4 (1,5% v/v) and A5 (2% v/v). Data were obtained using analysis of variance and
continued by Duncan New Multiple Range Test at level 5%. The result showed that the treatments significantly
affected on water content, solubility, resistance of water, and water vapour transmission rate. Edible film with
additional of essential oil inhibit the growth of Staphylococcus aureus FNCC-19 and Escherichia coli
FNCC-15. The best treatment was A5 (2% of essential oil) with thickness 0,156 mm, water content 22,87%,
solubility 30,82%, resistance of water 161,29%, water vapour transmission rate 0,291 g/m2/h, diameter
inhibition 20.16 mm of Staphylococcus aureus FNCC-19, and 30.45 mm of Escherichia coli FNCC-15.