HEMATOLOGICAL RESPONSE OF Pangasianodon hypophthalmus FED Cosmos caudatus ENRICHED FEED AND REARED IN SALINE MEDIA

Authors

  • Herliza Khairani Sahputri Department of Aquatic Resources Management, Faculty of Fisheries and Marine, Universitas Riau Author
  • Windarti Windarti Department of Aquatic Resources Management, Faculty of Fisheries and Marine, Universitas Riau Author
  • Deni Efizon Department of Aquatic Resources Management, Faculty of Fisheries and Marine, Universitas Riau Author
  • Ronal Kurniawan Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau Author
  • Okta Rizal Karsih Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau Author
  • Mega Novia Putri Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau Author

DOI:

https://doi.org/10.31258/ajoas.8.1.128-137

Keywords:

Salinity, Fish Blood, Feed Additive, Natural Herbs, Striped Catfish

Abstract

Raising striped catfish (Pangasianodon hypophthalmus) in dark conditions with low salinity (5 ppt) can support growth but may also cause stress and affect blood health. Adding Cosmos caudatus leaves, which contain natural bioactive compounds, in fish feed could offer a natural way to help boost the immune system. This study aimed to understand the hematological response of fish toward the C.caudatus enriched feed provision. The fish were reared for 60 days in 5 ppt salinity water and under a controlled photoperiod. There were four treatments: control (no C.caudatus) T1, T2 and T3 (10 g, 20 g, and 30 g of C.caudatus leave powder in 1 kg of feed). Parameters observed were red and white blood cell number, hemoglobin, hematocrit and glucose concentration, phagocytic activity, leukocrit, and types of white blood cells. Results showed that the T1 group significantly improved the ability of red blood cells, hemoglobin, hematocrit, and phagocytosis. Survival rates were high and showed no significant differences between treatments, suggesting that C.caudatus up to 30 g/kg is safe. Based on these findings, a dose of 10 g/kg can be recommended as an effective natural feed additive to help improve the health and immune system of P.hypopthalmus cultured in controlled rearing conditions

Downloads

Download data is not yet available.

References

1. Windarti, W., Siregar, S.H., Simarmata, A.H. Buku Teknologi Tepat Guna: Teknologi Budidaya Ikan dengan Manipulasi Fotoperiod, Sistem Akuaponik, dan Pemberian Pakan Mengandung Kelor (Moringa oliefera). CV. Eureka Media Aksara. Purbalingga, 2022.

2. Windarti, R., Riauwaty, M., Putra, R.M., Simarmata, A.H. Biological Aspects of Pangasianodon hypophthalmus Reared under Controlled Photoperiod. IOP Conference Series: Earth and Environmental Science, 2019; 348(1): 012050

3. Magwa, R.J., Windarti, W., Siregar, M.R. Pengaruh Manipulasi Fotoperiod dan Pakan yang Diperkaya Kunyit terhadap Pertumbuhan Ikan Patin (Pangasianodon hypophthalmus). Jurnal Ruaya, 2020; 8(2).

4. Alminiah, A. Pengendalian Ektoparasit pada Benih Ikan Mas (Cyprinus carpio L.) dengan Penambahan Garam Dapur (NaCl) di Balai Benih Perikanan Plalangan Kalisat Kabupaten Jember. Universitas Jember, 2015.

5. Riswan, M., Syawal, H., Tang, U.M. Osmotic Performance and Growth Rate of Striped Catfish (Pangasianodon hypophthalmus) at Different Salinity. Asian Journal of Aquatic Sciences, 2022; 5(1): 10-19.

6. Pamungkas, W. Aktivitas Osmoregulasi, Respons Pertumbuhan dan Energetic Cost pada Ikan yang Dipelihara dalam Lingkungan Bersalinitas. Media Akuakultur, 2012; 7(1): 44-51

7. Royan, F., Rejeki, S., Haditomo, A.H.C. Pengaruh Salinitas yang Berbeda terhadap Profil Darah Ikan Nila (Oreochromis niloticus). Journal of Aquaculture Management and Technology, 2014; 3(2): 109-117.

8. Riauwaty, M., Syawal, H. Gambaran Darah Ikan Nila (Orechromis niloticus) di Kolam Budidaya di Kecamatan Marpoyan Damai Kota Pekanbaru. Jurnal Perikanan dan Kelautan, 2016; 21(1): 1 – 6

9. Witeska, M., Kondera, E., Ługowska, K., Bojarski, B. Hematological Methods in Fish–Not Only for Beginners. Aquaculture, 2022; 547: 737498

10. Widiyantoro, A., Harlia, H. Antioxidant Activity of Leaves Extract of Kenikir (Cosmos caudatus Kunth) with Various Extraction Methods. Indonesian Journal of Pure and Applied Chemistry, 2020; 3(1): 9-20

11. Effendi, H. Telaah Kualitas Air Bagi Pengelolaan Sumberdaya dan Lingkungan Perairan. Kanisius. Yogyakarta, 2003.

12. Wandi, E. Deskripsi Hematologi Ikan Kelemak (Lebtobarbus hoevenii) yang Dikultur di dalam Keramba. Fakultas Perikanan dan Ilmu Kelautan. Universitas Riau. Pekanbaru, 2009.

13. Allifuddin, M. Peran Imunostimulan (Lipoolisakarida, Saccharomyces cerevisiae dan Levamiso) pada Gambaran Imunitas Ikan Jambal Siam (Pangasianodon hypothalmus). Program Pasca Sarjana IPB. Bogor, 1999; 48 p.

14. Ridwan, M., Lukistyowati, I., Syawal, H. Hematologis Eritrosit Ikan Patin Siam (Pangasius hypophthalmus) yang Diberi Pakan dengan Penambahan Larutan Biji Mangga Harumanis (Mangifera indica L.). Jurnal Ruaya, 2020; 8(2).

15. [SNI] Standar Nasional Indonesia. Metode Pengukuran Kualitas Air. Dinas Pekerjaan Umum. Jakarta, 1990.

16. Maftuch, H., Nursyam, N., Sukarni, S. Kajian Penggunaan Ciprofloxacin terhadap Hematologi Ikan Botia (Botia macracanthus) yang Diinfeksi Bakteri Aeromonas hydrophila. J. Exp. Life Sci., 2012; 2(2): 65-69.

17. Fauzan, M., Rosmaidar, S., Zuhrawati, M., Azhar, A. Pengaruh Tingkat Paparan Timbal (Pb) Terhadap Profil Darah Ikan Nila (Oreochromis niloticus). JIMVET, 2017; 1(4): 702-708.

18. Wahjuningrum, D., Ashry, N., Nuryati, S. Pemanfaatan Ekstrak Daun Ketapang (Terminalia cattapa) untuk Pencegahan dan Pengobatan Ikan Patin (Pangasianodon hypophthalmus) yang Terinfeksi Aeromonas hydrophila. Jurnal Akuakultur Indonesia, 2008; 7(1): 79-94.

19. Wang, M.Y., West, B.J., Jensen, C.J., Nowicki, D., Su, C., Palu, A.K. Anderson, G., Morinda citrifolia (Noni): A Literature Review and Recent Advances in Noni Research, Acta Pharmacol Sin., 2002; 23 (12): 1127- 1141.

20. Minaka, A., Sarjito, S., Hastuti, S. Identifikasi Agensia Penyebab dan Profil Darah Ikan Gurami (Osphronemus gouramy) yang erserang Penyakit Bakteri. Journal of Aquaculture Management and Technology, 2012; 1(1): 249-263.

21. Kuswardani, Y. Pengaruh Pemberian Resin Lebah terhadap Gambaran Darah Maskoki (Carassius Auratus) Yang Terinfeksi Bakteri Aeromonas hydrophila. Program Studi Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Bogor, 2006.

22. Fujaya, Y. Fisiologi Ikan: Dasar Pengembangan Teknologi Perikanan. Rineka Cipta. Jakarta, 179 p.

23. Noercholis, A., Muslim, A.Z., Maftuch, M. Ekstraksi Fitur Roundness untuk Menghitung Jumlah Leukosit dalam Citra Sel Darah Ikan. Jurnal EECCIS, 2013; 7(1): 35-40.

24. Putri, R.R., Basuki, F., Hastuti, S. Profil Darah dan Kelulushidupan Ikan Nila Pandu F5 (Oreochromis niloticus) yang Diinfeksi Bakteri Streptococcus agalactiae dengan Kepadatan Berbeda. Journal of Aquaculture Management and Technology, 2013; 2(2): 47-56

25. Suseno, D.N., Puspitasari, I., Jayanti, S. Efektivitas Probiotik terhadap Efisiensi Pakan dan Ulas Darah Ikan Komet (Carassius auratus). Grouper: Jurnal Ilmiah Perikanan, 2022; 13(2): 184-190

26. Purnomo, D., Sugiharto, S., Isroli, I. Total Leukosit dan Diferensial Leukosit Darah Ayam Broiler akibat Penggunaan Tepung Onggok Fermentasi Rhizopus oryzae pada Ransum. Jurnal Ilmu-Ilmu Peternakan, 2015; 25(3): 59-68

27. Rahardjo, M.F., Sjafei, D.S., Affandi, R., Sulistiono, S. Ikhtiologi. Lubuk Agung. Jakarta, 2011.

28. Kusuma, I.J., Prasetyorini, P., Wardatun, S. Toksisitas Ekstrak Daun Kenikir (Cosmos caudatus Kunth) dengan Perbedaan Metode dan Jenis Pelarut Berbeda. JOM Bidang Farmasi Universitas Pakuan, 2018; 1(1).

29. Djauhari, R., Matling, M., Monalisa, S.S., Sianturi, E. Respon Glukosa Darah Ikan Betok (Anabas testudineus) terhadap Stres Padat Tebar. Jurnal Ilmu Hewani Tropika (Journal of Tropical Animal Science), 2020; 8(2): 43-49.

30. Lukistyowati, I. Hematologi Ikan-ikan Air Tawar. Lembaga Penelitian Universitas Riau. Pekanbaru, 2007.

31. Robert, R.J. Fish Pathology. Wiley-Blackwell. Iowa, 2012.

32. Lusiastuti, A.M., Sumiati, T., Hadie, E. Probiotik Bacillus firmus untuk Pengendalian Penyakit Aeromonas hydrophila pada Budidaya Ikan Lele Dumbo (Clarias gariepinus). Jurnal Riset Akuakultur, 2013; 8(2): 253-264.

33. Juharni, J., Muchdar, F. Peningkatan Aktivitas Fagositosis pada Ikan Kerapu Bebek (Cromileptes altivelis) dengan Pemberian Immunostimulan (ᵦ-Glucan) yang Diekstrak dari Jamur Tiram Putih (Lerotus ostreatus). Prosiding Seminar Nasional Kemaritiman dan Sumberdaya Pulau-pulau Kecil, 2017; 1(1): 62-70

34. Mahyuddin, K. Panduan Lengkap Agribisnis Patin. Penebar Swadaya. Jakarta, 2010; 212 p.

35. Kordi, H.G. Pengelolaan Kualitas Air dalam Budidaya Perairan. PT Citra Aditya Bakti. Bandung, 2005; 964 p.

Downloads

Published

2025-05-04

Issue

Vol. 8 No. 1 (2025): April

Section

Articles

License

Copyright (c) 2025 Herliza Khairani Sahputri, Windarti Windarti, Deni Efizon, Ronal Kurniawan, Okta Rizal Karsih, Mega Novia Putri (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.